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Image Search Results
Journal:
Article Title: Comparison of GenFlex Tag Array and Pyrosequencing in SNP Genotyping
doi:
Figure Lengend Snippet: Reaction chemistry of the 3 SNP genotyping systems tested. A: In solid-phase minisequencing, PCR is performed using biotinylated primers. The biotinylated PCR product is captured to a streptavidin (Strep)-coated, solid-phase (eg, microtitration plate), and denatured. The unbound strand is washed away from the reaction solution. A single nucleotide extension reaction using 3H deoxynucleotides is performed on the captured, single-stranded template. B: In Pyrosequencing, the PCR is performed using biotinylated primers and captured to a solid-phase (beads), similar to minisequencing. Yet the beads provide a much greater binding capacity than streptavidin-coated microtitration plates. The unbound strand is washed away. A four enzyme sequencing reaction, for a stretch of 4 to 5 consecutive nucleotides, is performed. Each nucleotide extension releases pyrophosphate. C: In the Tag-Array format, the PCR is performed using regular primers, but the denatured PCR product is captured by hybridization to oligonucleoties which have a Tag sequence as a tail. This tail sequence is complementary to a specific oligonucleotide Tag’s on the array. The primer extension reaction is performed using differently labeled fluorescent dideoxynucleotides.
Article Snippet: Pyrosequencing
Techniques: Binding Assay, Sequencing, Hybridization, Labeling
Journal:
Article Title: Comparison of GenFlex Tag Array and Pyrosequencing in SNP Genotyping
doi:
Figure Lengend Snippet: Comparison of Features of the Three SNP Genotyping Systems
Article Snippet: Pyrosequencing
Techniques: Comparison, Multiplex Assay, Microarray, Software